| 1. | Genetic transform ation of al hagi preudoalhagi with agrobacterium rhizogenes a
4对骆驼刺的遗传转化 |
| 2. | Transformation of glycyrrhiza inflat bat by agrobacterium rhizogenes r
发根农杆菌转化胀果甘草的研究 |
| 3. | Studies on factors affecting the transformation of astragalus mombranaceus bunge var . mongholicus with agrobacterium rhizogenes
质粒转化蒙古黄芪影响因素的研究 |
| 4. | This paper reports hairy roots induced by agrobacterium rhizogenes and production of regenerative plants from hairy root cultures in maize
本文利用发根农杆菌遗传转化玉米，通过毛状根的诱导、毛状根的分化培养获得再生植株。 |
| 5. | But the concentration of a4 at 40 h was higher than that of other strains . four strains ( a4 , r1205 , r1000 , r1601 ) in this study were agrobacterium rhizogenes according to pcr assay
利用pcr方法鉴定结果表明，所有供试菌株中， a4 、 r1205 、 r1000和r1601为含ri质粒的发根农杆菌，而r1022和15834未出现阳性结果。 |
| 6. | 2 establishment of genetic transformation system by agrobacterium rhizogenes the different types of explants ( hypocotyls , cotyledon , leaf blade , petiole ) were infected by strain r1601 of agrobacterium rhizogenes
（ 2 0mg l ）上培养30d后经过体细胞胚胎发生途径产生大量的丛生苗；子叶培养在诱导子叶产生丛生苗培养基（ msoc ） ms ba （ 2 0 。 |
| 7. | Agrobacterium rhizogenes ( rhizobiacease ) is a kind of gram - negative bacterium , which contains a big ri plasmid ( 200kb ) - root inducing plasmid . there are three types of ri plasmid , mannopine type , cucumopine type and agropine type , according to the type of opine synthesized in transgenic cell
根据转化细胞合成的冠瘿碱( opine )的类型不同， ri质粒分为3种类型：甘露碱型( mannopinetype ) 、黄瓜碱型( cucumopinetype )和农杆碱型( agropinetype ) 。 |
| 8. | The purpose of this study is to provide theoretical and technical support for torenia transgenic hairy root and other molecular researches used by torenia . the comparation and identification of agrobacterium rhizogenes strains were investigated using 3 - ketolactose test , the differential acid production assay and the motility assay
采用3 -酮乳糖产物法、差异酸生成实验和游动性实验鉴定6种发根农杆菌供试菌株a4 、 r1205 、 r1000 、 r1601 、 r1022和15834 。 |
| 9. | Tissue culture and plantlet regeneration from leaf explants of a . ficoidea cv . " ruliginosa " and its genetic transformation with agrobacterium rhizogenes atcc 15834 were investigated as well as changes in the contents of sucrose , nitrogen and phosphate in liquid medium during the hairy root cultures
本文研究了红龙草( altemantheraficoideacv " ruliginosa " )叶片的组织培养、发根农杆菌( agrobacteriumrhizogenes ) atcc15834对红龙草的遗传转化以及毛状根液体培养过程中培养液某些营养成分的消耗变化。 |
| 10. | The transformed hairy roots were detected for agropine to make sure the transformation was successful . a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ) . od600 = 0 . 7 was the optimum infection concentration for the tested transforming agent , with temperature at 25 , ph at 6 . 0 , 20min of infection and 48h of co - culture and phytohormone at 1 . 0mg / l being the most favourable hairy root - inducting pattern
通过实验和对结果的数据分析，得出下面的结论：发根农杆菌r1601是较好的大苞栝楼发根的诱导菌株，它对预培养24h的大苞栝楼子叶外植体具有最好的转化效率和发根诱导率( 60 ) ，最适的菌株感染浓度od _ ( 600 )为0 . 7 ，温度27 、 ph值为6 . 0 、 20min的感染时间和48h的共培养，以及黑暗条件和1 . 0mg / l的激素最有利于发根诱导的环境条件。 |
